Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (37ug/lane). Beta-actin (arrow) was detected using the purified Mab.
SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
76AT630.91.31
Host Species
Mouse
Tested Applications
WB
WB:1:100~500
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
SMT3B SMT3H1
Alternative Names
SUMO3 SMT3B SMT3H1 Small ubiquitin-related modifier 3 SMT3 homolog 1 SUMO-2 Ubiquitin-like protein SMT3B
Molecular Weight(MW)
11637 Da
Function
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4
Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (37ug/lane). Beta-actin (arrow) was detected using the purified Mab.
Application Notes
WB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.