Application
TYSY Antibody (C-term) (Cat. #AX10003) western blot analysis in Hela,Jurkat and HL-60 cell line lysates (35ug/lane).This demonstrates the TYSY antibody detected the TYSY protein (arrow).Application
TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TYSY Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
Confocal immunofluorescent analysis of TYSY Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
TYSY Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TYSY (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYMS; TS; Thymidylate synthase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Clone Number | RB20041 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TS |
|---|---|
| Alternative Names | TYMS TS Thymidylate synthase |
| Molecular Weight(MW) | 35716 Da |
| Function | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway |
| Tissue Specificity | This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~302 amino acids from the C-terminal region of human TYSY. |
| Cellular Localization | Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane |
| Entrez Gene | 7298 |
|---|
Application
TYSY Antibody (C-term) (Cat. #AX10003) western blot analysis in Hela,Jurkat and HL-60 cell line lysates (35ug/lane).This demonstrates the TYSY antibody detected the TYSY protein (arrow).
Application
TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TYSY Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYSY Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TYSY Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
Confocal immunofluorescent analysis of TYSY Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
TYSY Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
