WB
Western blot analysis of extracts from mouse brain/rat brain, using Phospho-CACNA1C (Ser1981) Antibody.IHC
Phospho-CACNA1C (Ser1981) Antibodyat 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-CACNA1C (Ser1981) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CACNA1C around the phosphorylation site of Ser1981. |
| Modification | p-Ser1981 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CACNA1C |
|---|---|
| Gene Synonyms | TS LQT8 CACH2 CACN2 CaV1.2 CCHL1A1 NEDHLSS CACNL1A1 TS. LQT8 CACNA1C-IT2 |
| Gene Full Name | calcium voltage-gated channel subunit alpha1 C |
| Gene Summary | This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012] |
| Alternative Names | alpha-1 polypeptide cardiac muscle isoform 1 L type CAC1C_HUMAN CACH 2 CACH2 CACN 2 CACN2 CACNA1C CACNL1A1 Calcium channel Calcium channel cardic dihydropyridine sensitive alpha 1 subunit Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle Calcium channel voltage dependent L type alpha 1C subunit CaV1.2 CCHL1A1 DHPR alpha 1 DHPR alpha 1 subunit LQT8 TS Voltage dependent L type calcium channel alpha 1C subunit Voltage dependent L type calcium channel subunit alpha 1C Voltage gated calcium channel alpha subunit Cav1.2 Voltage gated calcium channel subunit alpha Cav1.2 Voltage gated L type calcium channel Cav1.2 alpha 1 subunit splice variant 10* Voltage-dependent L-type calcium channel subunit alpha-1C Voltage-gated calcium channel subunit alpha Cav1.2 |
| Molecular Weight(MW) | 249kDa |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma>Multi-pass membrane protein. Perikaryon. Cell junction>Synapse>Postsynaptic density membrane. Cell projection>Dendrite. Cell membrane>Sarcolemma>T-tubule. Note: Colocalizes with ryanodine receptors in distinct clusters at the junctional membrane, where the sarcolemma and the sarcoplasmic reticulum are in close contact. The interaction between RRAD and CACNB2 promotes the expression of CACNA1C at the cell membrane. |
WB
Western blot analysis of extracts from mouse brain/rat brain, using Phospho-CACNA1C (Ser1981) Antibody.
IHC
Phospho-CACNA1C (Ser1981) Antibodyat 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
