Application
Western blot analysis of anti-UBCH9 Antibody (N-term) in Y79 cell line lysates (35ug/lane). UBCH9 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | UBCH9 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2E3; UBCE4; UBCH9; Ubiquitin-conjugating enzyme E2 E3; UbcH9; Ubiquitin carrier protein E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBCE4 UBCH9 |
|---|---|
| Alternative Names | UBE2E3 UBCE4 UBCH9 Ubiquitin-conjugating enzyme E2 E3 UbcH9 Ubiquitin carrier protein E3 Ubiquitin-conjugating enzyme E2-23 kDa Ubiquitin-protein ligase E3 |
| Molecular Weight(MW) | 22913 Da |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest |
| Tissue Specificity | Ubiquitously expressed at low levels. Highly expressed in skeletal muscle |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner |
| Entrez Gene | 10477 |
|---|
Application
Western blot analysis of anti-UBCH9 Antibody (N-term) in Y79 cell line lysates (35ug/lane). UBCH9 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
