Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest
Tissue Specificity
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle
Cellular Localization
Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner
The anti-UBCH9 Pab is used in Western blot to detect UBCH9 in Jurkat cell lysate.
Application Notes
WB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.