Application
Western blot analysis of ZBTB7B Antibody (C-term) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab (1:60 dilution).Application
Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term)(Cat#AP6370b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.| Product Name | ZBTB7B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZBTB15, ZFP67, ZNF857B |
|---|---|
| Alternative Names | ZBTB7B ZBTB15 ZFP67 ZNF857B Zinc finger and BTB domain-containing protein 7B Krueppel-related zinc finger protein cKrox T-helper-inducing POZ/Krueppel-like factor Zinc finger and BTB domain-containing protein 15 Zinc finger protein 67 homolog Zinc |
| Molecular Weight(MW) | 58027 Da |
| Function | Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (By similarity) |
| Tissue Specificity | This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447~477 amino acids from the C-terminal region of human ZBTB7B. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 51043 |
|---|
Application
Western blot analysis of ZBTB7B Antibody (C-term) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab (1:60 dilution).
Application
Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term)(Cat#AP6370b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
