Application
Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALOX12 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LOG12 |
|---|---|
| Alternative Names | ALOX12 LOG12 Arachidonate 12-lipoxygenase 12S-type Platelet-type lipoxygenase 12 |
| Molecular Weight(MW) | 75694 Da |
| Function | Oxygenase and 14,15-leukotriene A4 synthase activity. |
| Tissue Specificity | This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627~656 amino acids from the C-terminal region of human ALOX12 . |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 239 |
|---|
Application
Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
