WB
12-LO:Westernblotanalysisof12-LO expressioninnon-transfected293T:Mouse Monoclonal antibody to 12-LO(A), mouse12-LOtransfected293T:Mouse Monoclonal antibody to 12-LO(B)whole cell lysatesandhumanplateletextract (C).ICC/IF
12-LO: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to 12-LO |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 131-230 mapping within an internal region of 12-LO of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALOX12 |
|---|---|
| Gene Synonyms | LOG12 12-LOX 12S-LOX |
| Gene Full Name | arachidonate 12-lipoxygenase, 12S type |
| Gene Summary | This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 76 kDa |
| Cellular Localization | Cytoplasm,Membrane. |
WB
12-LO:Westernblotanalysisof12-LO expressioninnon-transfected293T:Mouse Monoclonal antibody to 12-LO(A), mouse12-LOtransfected293T:Mouse Monoclonal antibody to 12-LO(B)whole cell lysatesandhumanplateletextract (C).
ICC/IF
12-LO: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
