Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Immunofluorescent analysis of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).Application
Western blot analysis of ALOX12 Antibody in K562 cell line lysates (35ug/lane)| Product Name | ALOX12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12 |
| Product description | Oxygenase and 14,15-leukotriene A4 synthase activity.1) Yoshimoto,T., et.al., Biochem. Biophys. Res. Commun. 172 (3), 1230-1235 (1990) |
| Immunogen | This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ALOX12 |
|---|---|
| Alternative Names | Arachidonate 12-lipoxygenase 12S-type 12S-LOX 12S-lipoxygenase Lipoxin synthase 12-LO 332- Platelet-type lipoxygenase 12 ALOX12 12LO LOG12 |
| Molecular Weight(MW) | 76 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Immunofluorescent analysis of A549 cells, using ALOX12 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Application
Western blot analysis of ALOX12 Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
