WB
Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, All lanes: ALOX12 antibody at 3.53µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of ALOX12 Antibody diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IHC
IHC image of ALOX12 Antibody diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of A549 cells with ALOX12 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-ALOX12 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Arachidonate 12-lipoxygenase, 12S-type protein (508-658AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ALOX12 |
|---|---|
| Gene Synonyms | LOG12 12-LOX 12S-LOX |
| Gene Full Name | arachidonate 12-lipoxygenase, 12S type |
| Gene Summary | This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | 12 LOX antibody 12(S) lipoxygenase antibody 12-lipoxygenase antibody 12LO antibody 12S LOX antibody 12S-lipoxygenase antibody 12S-LOX antibody 12S-type antibody Alox12 antibody Arachidonate 12 lipoxygenase antibody Arachidonate 12 lipoxygenase 12S type antibody Arachidonate 12-lipoxygenase antibody Arachidonate 12-oxidoreductase antibody Lipoxin synthase 12-LO antibody LOG12 antibody LOX12_HUMAN antibody P-12LO antibody Platelet 12-LOX antibody Platelet type lipoxygenase 12 antibody platelet-type 12-lipoxygenase antibody Platelet-type lipoxygenase 12 antibody |
| Molecular Weight(MW) | 76kDa |
| Cellular Localization | Cytoplasm, cytosol. Membrane. |
WB
Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, All lanes: ALOX12 antibody at 3.53µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of ALOX12 Antibody diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
IHC image of ALOX12 Antibody diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of A549 cells with ALOX12 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
