Application
Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.Application
ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ALOX12 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ALOX12 (C-term)Oxygenase and 14,15-leukotriene A4 synthase activity. |
| Immunogen | KLH conjugated synthetic peptide between 627~656 amino acids from the C-terminal region of human ALOX12 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | ALOX12 Platelettype lipoxygenase 12 Arachidonate 12-lipoxygenase 12S-type 12S-lipoxygenase Arachidonate 12lipoxygenase 12Stype 12Slipoxygenase Platelettype lipoxygenase12 Platelet-type lipoxygenase 12 ALOX12 LOG12 12SLOX Arachidonate12lipoxyge |
|---|---|
| Target | ALOX12 |
| Molecular Weight(MW) | 75.694 kDa (note) |
Application
Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.
Application
ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of ALOX12 Antibody (C-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
