Application
A. Western blot analysis on HeLa cell lysate using anti-Phospho-ATF-2 (pT71) RabMAb (Cat. #AJ1064c); dilution 1:10,000. Cells were (1) untreated, and (2) treated with Anisomycin.Application
B. Immunofluorescent staining of HeLa cells using anti-Phospho-ATF-2 (pT71) RabMAb (Cat. #AJ1064c).| Product Name | ATF-2 Antibody Phospho (pT71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T71 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E268 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CREB2, CREBP1 |
|---|---|
| Alternative Names | ATF2 CREB2 CREBP1 Cyclic AMP-dependent transcription factor ATF-2 Activating transcription factor 2 cAMP response element-binding protein CRE-BP1 HB16 Cyclic AMP-responsive element-binding protein 2 |
| Molecular Weight(MW) | 54537 Da |
| Function | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex |
| Tissue Specificity | Abundant expression seen in the brain. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1386 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Phospho-ATF-2 (pT71) RabMAb (Cat. #AJ1064c); dilution 1:10,000. Cells were (1) untreated, and (2) treated with Anisomycin.
Application
B. Immunofluorescent staining of HeLa cells using anti-Phospho-ATF-2 (pT71) RabMAb (Cat. #AJ1064c).| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
