Application
Western blot analysis of anti-APG7 Monoclonal Antibody (Cat.#AM1813a) by Recombinant APG7 protein (Fragment 34KD). APG7 protein (Fragment 34KD)(arrow) was detected using the ascites Mab.| Product Name | ATG7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 29CT10.3.12 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG7L |
|---|---|
| Alternative Names | ATG7 APG7L Ubiquitin-like modifier-activating enzyme ATG7 ATG12-activating enzyme E1 ATG7 Autophagy-related protein 7 Ubiquitin-activating enzyme E1-like protein |
| Molecular Weight(MW) | 77960 Da |
| Function | Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity) |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 10533 |
|---|
Application
Western blot analysis of anti-APG7 Monoclonal Antibody (Cat.#AM1813a) by Recombinant APG7 protein (Fragment 34KD). APG7 protein (Fragment 34KD)(arrow) was detected using the ascites Mab.| Application Notes | WB:1:500~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
