Application
Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in HepG2, 293, Jurkat and Hela cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution).Application
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).Application
Breast CA section stained with Autophagy Beclin 1 Antibody (Cat. # AM1818a) at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana.| Product Name | Beclin 1 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 15CT26 |
| Host Species | Mouse |
| Tested Applications | IFWBIHC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GT197 |
|---|---|
| Alternative Names | BECN1 GT197 Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 |
| Molecular Weight(MW) | 51896 Da |
| Function | Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) |
| Entrez Gene | 8678 |
|---|
Application
Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in HepG2, 293, Jurkat and Hela cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution).
Application
Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).
Application
Breast CA section stained with Autophagy Beclin 1 Antibody (Cat. # AM1818a) at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
