Application
CALR Antibody western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the CALR antibody detected CALR protein (arrow).Application
CALR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CALR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CALR; CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; HACBP; grp60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRTC |
|---|---|
| Alternative Names | CALR CRTC Calreticulin CRP55 Calregulin Endoplasmic reticulum resident protein 60 HACBP grp60 |
| Molecular Weight(MW) | 48142 Da |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity) |
| Tissue Specificity | This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR. |
| Cellular Localization | Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen (By similarity). Note=Also found in cell surface (T cells), cytosol and extracellular matrix Associated with the lytic granules in the cytolytic T-lymphocytes |
| Entrez Gene | 811 |
|---|---|
| Nucleotide Accession | NP_004334.1 |
Application
CALR Antibody western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the CALR antibody detected CALR protein (arrow).
Application
CALR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
