Application
Western blot analysis of CALR Antibody (Center) in Ramos, Jurkat, HepG2, NIH-3T3 cell line lysates (35ug/lane). CALR (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CALR Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CALR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CALR; CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; HACBP; grp60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRTC |
|---|---|
| Alternative Names | CALR CRTC Calreticulin CRP55 Calregulin Endoplasmic reticulum resident protein 60 HACBP grp60 |
| Molecular Weight(MW) | 48142 Da |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity) |
| Tissue Specificity | This CALR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~313 amino acids from the Center region of human CALR. |
| Cellular Localization | Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen (By similarity). Note=Also found in cell surface (T cells), cytosol and extracellular matrix Associated with the lytic granules in the cytolytic T-lymphocytes |
| Entrez Gene | 811 |
|---|
Application
Western blot analysis of CALR Antibody (Center) in Ramos, Jurkat, HepG2, NIH-3T3 cell line lysates (35ug/lane). CALR (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CALR Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
