Application
Fig 1. Western blot analysis on (A) SH-SY5Y, (B) HL-60, (C) HepG2, (D) HeLa, (E) Fetal kidney and (F) Fetal brain lysates using anti-Calreticulin RabMAb (Cat. #AJ1124a).Application
Fig 3. Immunofluorescent staining of HeLa cells using anti-Calreticulin RabMAb (Cat. #AJ1124a).Application
Fig 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin RabMAb (Cat. #AJ1124a).| Product Name | Calreticulin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3924 |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRTC |
|---|---|
| Molecular Weight(MW) | 48142 Da |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Calreticulin was used as an immunogen. |
| Cellular Localization | Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen (By similarity). Note=Also found in cell surface (T cells), cytosol and extracellular matrix Associated with the lytic granules in the cytolytic T-lymphocytes |
| Entrez Gene | 811 |
|---|
Application
Fig 1. Western blot analysis on (A) SH-SY5Y, (B) HL-60, (C) HepG2, (D) HeLa, (E) Fetal kidney and (F) Fetal brain lysates using anti-Calreticulin RabMAb (Cat. #AJ1124a).
Application
Fig 3. Immunofluorescent staining of HeLa cells using anti-Calreticulin RabMAb (Cat. #AJ1124a).
Application
Fig 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin RabMAb (Cat. #AJ1124a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
