Application
CD44 antibody (Cat. #AM1901b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD44 antibody detected the CD44 protein (arrow).Application
CD44 antibody (Cat. #AM1901b) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
CD44 antibody (Cat. #AM1901b) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).Application
CD44 Antibody (Cat. #AM1901b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | CD44 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | Hermes-3 |
| Host Species | Mouse |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LHR, MDU2, MDU3, MIC4 |
|---|---|
| Alternative Names | CD44 LHR MDU2 MDU3 MIC4 CD44 antigen CDw44 Epican Extracellular matrix receptor III GP90 lymphocyte homing/adhesion receptor HUTCH-I Heparan sulfate proteoglycan Hermes antigen Hyaluronate receptor Phagocytic glycoprotein 1 Phagocytic glycop |
| Molecular Weight(MW) | 81538 Da |
| Function | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events |
| Tissue Specificity | Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas Expression is repressed in neuroblastoma cells |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges (By similarity) |
| Entrez Gene | 960 |
|---|---|
| Nucleotide Accession | NP_000601.3 |
Application
CD44 antibody (Cat. #AM1901b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD44 antibody detected the CD44 protein (arrow).
Application
CD44 antibody (Cat. #AM1901b) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CD44 antibody (Cat. #AM1901b) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).
Application
CD44 Antibody (Cat. #AM1901b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
