Application
Figure 1: Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).Application
Figure 2: Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.| Product Name | CD44 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8E2F3 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LHR, MDU2, MDU3, MIC4 |
|---|---|
| Alternative Names | IN LHR MC56 MDU2 MDU3 MIC4 Pgp1 CDW44 CSPG8 HCELL |
| Molecular Weight(MW) | 81538 Da |
| Function | Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events |
| Tissue Specificity | Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas Expression is repressed in neuroblastoma cells |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges (By similarity) |
| Entrez Gene | 960 |
|---|---|
| Nucleotide Accession | NP_001001392.1 |
Application
Figure 1: Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Application
Figure 2: Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli. |
