Application
Figure 1: Western blot analysis using anti-CIB1 monoclonal antiobdy against truncated CIB1 recombinant protein(1) and A431 cell lysate(2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human thalamus(left) and glioma(right) tissue, showing membrane localization using anti-CIB1 antibody with DAB staining.| Product Name | CIB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIB; KIP; KIP1; SIP2-28 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 5A1F5E12, 5A1H7E12 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CIB KIP PRKDCIP |
|---|---|
| Alternative Names | CIB KIP KIP1 SIP2-28 |
| Molecular Weight(MW) | 21703 Da |
| Function | May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes) Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Membrane; Lipid-anchor. Cytoplasm. Nucleus. Cell projection, filopodium. Apical cell membrane. Note=Localizes to the perinuclear region in the presence of NBR1. Colocalizes with TAS1R2 in apical regions of taste receptor cells |
| Entrez Gene | 10519 |
|---|---|
| Nucleotide Accession | NP_006375.2 |
Application
Figure 1: Western blot analysis using anti-CIB1 monoclonal antiobdy against truncated CIB1 recombinant protein(1) and A431 cell lysate(2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human thalamus(left) and glioma(right) tissue, showing membrane localization using anti-CIB1 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of CIB1 expressed in E. Coli. |
