Application
Figure 1: Western blot analysis using anti-CK18 monoclonal antibody against Hela (1), NIH/3T3 (2), A549 (3), Jurkat (4), MCF-7(5), HepG2 (6), A431 (7), HEK293 (8) and K562 (9) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), hepatocarcinoma(B), stomach cancer(C) and colon cancer tissue(D), showing cytoplasmic location with DAB staining using anti-CK18 Antibody.| Product Name | Cytokeratin 18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK18; CYK18; KRT18 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 4D11E4 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK18 |
|---|---|
| Alternative Names | CK18 CYK18 KRT18 |
| Molecular Weight(MW) | 48058 Da |
| Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection |
| Tissue Specificity | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma |
| Cellular Localization | Cytoplasm, perinuclear region. Nucleus, nucleolus |
| Entrez Gene | 3875 |
|---|---|
| Nucleotide Accession | NP_954657.1 |
Application
Figure 1: Western blot analysis using anti-CK18 monoclonal antibody against Hela (1), NIH/3T3 (2), A549 (3), Jurkat (4), MCF-7(5), HepG2 (6), A431 (7), HEK293 (8) and K562 (9) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), hepatocarcinoma(B), stomach cancer(C) and colon cancer tissue(D), showing cytoplasmic location with DAB staining using anti-CK18 Antibody.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli. |
