Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-CK-8 (pS23) RabMAb (Cat. #AJ1230d), 1:50,000 dilution. Cells were either (A) untreated (B) treated with CalyculinApplication
B. Positive immunohistochemical staining of paraffin-embedded human gastric adenocarcinoma using anti-CK-8 Phospho-RabMAb (Cat. #AJ1230d)Application
C. Positive immunohistochemical staining of paraffin-embedded human breast adenocarcinoma using anti-CK-8 Phospho- RabMAb (Cat. #AJ1230d).| Product Name | Cytokeratin-8 Antibody Phospho (pS23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT8, CYK8, Keratin, type II cytoskeletal 8, Keratin-8 |
| Modification | p-S23 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1629Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK8 |
|---|---|
| Alternative Names | KRT8 CYK8 Keratin type II cytoskeletal 8 Keratin-8 |
| Molecular Weight(MW) | 53704 Da |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm (By similarity). Nucleus matrix (By similarity) |
| Entrez Gene | 3856 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-CK-8 (pS23) RabMAb (Cat. #AJ1230d), 1:50,000 dilution. Cells were either (A) untreated (B) treated with Calyculin
Application
B. Positive immunohistochemical staining of paraffin-embedded human gastric adenocarcinoma using anti-CK-8 Phospho-RabMAb (Cat. #AJ1230d)
Application
C. Positive immunohistochemical staining of paraffin-embedded human breast adenocarcinoma using anti-CK-8 Phospho- RabMAb (Cat. #AJ1230d).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
