Application
Figure 1: Western blot analysis using anti-CK8 monoclonal antibody against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), lung cancer(B) and ovarian cancer tissue(C), showing membrane and cytoplasmic localization with DAB staining using anti-CK8 antibody.Application
Figure 3: Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using anti-CK8 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | Cytokeratin 8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK8; CYK8; K2C8; KRT8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8A5D12 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK8 |
|---|---|
| Alternative Names | CK8 CYK8 K2C8 KRT8 |
| Molecular Weight(MW) | 53704 Da |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm (By similarity). Nucleus matrix (By similarity) |
| Entrez Gene | 3856 |
|---|---|
| Nucleotide Accession | NP_002264.1 |
Application
Figure 1: Western blot analysis using anti-CK8 monoclonal antibody against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), lung cancer(B) and ovarian cancer tissue(C), showing membrane and cytoplasmic localization with DAB staining using anti-CK8 antibody.
Application
Figure 3: Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using anti-CK8 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli. |
