Application
A.Western blot analysis on HeLa lysates using anti-Phospho-Cytokeratin 8 (pS431) RabMAb (Cat. #AJ1230e). Cells were either (A) untreated (B) treated with FBS and Calyculin AApplication
B.Immunohistochemical analysis of (A) paraffin-embedded human ductal breast carcinoma using Phospho-specific anti-Cytokeratin 8 RabMAb (Cat. #AJ1230e)Application
C. Immunohistochemical analysis of paraffin-embedded human stomach adenocarcinoma using anti-Phospho-Cytokeratin 8 RabMAb (Cat. #AJ1230e).| Product Name | Cytokeratin-8 Antibody Phospho (pS431) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT8, CYK8, Keratin, type II cytoskeletal 8, Keratin-8 |
| Modification | p-S431 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1630 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK8 |
|---|---|
| Alternative Names | KRT8 CYK8 Keratin type II cytoskeletal 8 Keratin-8 |
| Molecular Weight(MW) | 53704 Da |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm (By similarity). Nucleus matrix (By similarity) |
| Entrez Gene | 3856 |
|---|
Application
A.Western blot analysis on HeLa lysates using anti-Phospho-Cytokeratin 8 (pS431) RabMAb (Cat. #AJ1230e). Cells were either (A) untreated (B) treated with FBS and Calyculin A
Application
B.Immunohistochemical analysis of (A) paraffin-embedded human ductal breast carcinoma using Phospho-specific anti-Cytokeratin 8 RabMAb (Cat. #AJ1230e)
Application
C. Immunohistochemical analysis of paraffin-embedded human stomach adenocarcinoma using anti-Phospho-Cytokeratin 8 RabMAb (Cat. #AJ1230e).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
