Application
A. Western blot analysis on A431 cell lysates using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f), dilution 1:10,000. Cell lysates were either (1) untreated or (2) treated with EGF.Application
B. Immunohistochemical analysis of paraffin-embedded liver carcinoma using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).Application
C. Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).| Product Name | Cytokeratin-8 Antibody Phospho (pS73) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT8, CYK8, Keratin, type II cytoskeletal 8, Keratin-8 |
| Modification | p-S73 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E431-2 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK8 |
|---|---|
| Alternative Names | KRT8 CYK8 Keratin type II cytoskeletal 8 Keratin-8 |
| Molecular Weight(MW) | 53704 Da |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm (By similarity). Nucleus matrix (By similarity) |
| Entrez Gene | 3856 |
|---|
Application
A. Western blot analysis on A431 cell lysates using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f), dilution 1:10,000. Cell lysates were either (1) untreated or (2) treated with EGF.
Application
B. Immunohistochemical analysis of paraffin-embedded liver carcinoma using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).
Application
C. Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
