Application
Western blot analysis of CDH1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDH1 (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of CDH1 (arrow) using rabbit polyclonal CDH1 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies).Application
Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | E Cadherin (CDH1) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDH1; CDHE; UVO; E-Cad/CTF3; Cadherin-1; CAM 120/80; Epithelial cadherin; Uvomorulin; CD_antigen=CD324; E-Cad/CTF1; E-Cad/CTF2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDHE UVO |
|---|---|
| Alternative Names | CDH1 CDHE UVO E-Cad/CTF3 Cadherin-1 CAM 120/80 Epithelial cadherin Uvomorulin CD_antigen=CD324 E-Cad/CTF1 E-Cad/CTF2 |
| Molecular Weight(MW) | 97456 Da |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 |
| Tissue Specificity | Non-neural epithelial tissues. |
| Cellular Localization | Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane |
| Entrez Gene | 999 |
|---|
Application
Western blot analysis of CDH1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CDH1 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of CDH1 (arrow) using rabbit polyclonal CDH1 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies).
Application
Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
