Application
A. Western blot analysis on MCF-7 cell lysate using anti-E-cadherin RabMAb (Cat. #AJ1249a), dilution 1:200,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-E-cadherin RabMAb (Cat. #AJ1249a).Application
C. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-E-cadherin RabMAb (Cat. #AJ1249a).| Product Name | E-Cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDH1, CDHE, UVO, E-Cad/CTF3, Uvomorulin;CAM 120/80 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP700Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDHE UVO |
|---|---|
| Alternative Names | CDH1 CDHE UVO E-Cad/CTF3 Uvomorulin CAM 120/80 |
| Molecular Weight(MW) | 97456 Da |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 |
| Tissue Specificity | Non-neural epithelial tissues. |
| Cellular Localization | Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane |
| Entrez Gene | 999 |
|---|
Application
A. Western blot analysis on MCF-7 cell lysate using anti-E-cadherin RabMAb (Cat. #AJ1249a), dilution 1:200,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-E-cadherin RabMAb (Cat. #AJ1249a).
Application
C. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-E-cadherin RabMAb (Cat. #AJ1249a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
