Application
EIF4E Antibody (Cat. #AM1852b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).Application
Confocal immunofluorescent analysis of EIF4E Antibody (Cat#AM1852b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | EIF4E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 163CT48.1.9 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF4EL1, EIF4F |
|---|---|
| Alternative Names | EIF4E EIF4EL1 EIF4F Eukaryotic translation initiation factor 4E eIF-4F 25 kDa subunit mRNA cap-binding protein |
| Molecular Weight(MW) | 25097 Da |
| Function | Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap |
| Tissue Specificity | This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein. |
| Cellular Localization | Cytoplasm, P-body. |
| Entrez Gene | 1977 |
|---|---|
| Nucleotide Accession | NP_001959.1 |
Application
EIF4E Antibody (Cat. #AM1852b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).
Application
Confocal immunofluorescent analysis of EIF4E Antibody (Cat#AM1852b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
