Application
Western blot analysis of EphB1 (arrow) using rabbit polyclonal EphB1 Antibody 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 gene (Lane 2) (Origene Technologies).| Product Name | EphB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ELK, EPHT2, HEK6, NET |
|---|---|
| Alternative Names | EPHB1 ELK EPHT2 HEK6 NET Ephrin type-B receptor 1 EPH tyrosine kinase 2 EPH-like kinase 6 Neuronally-expressed EPH-related tyrosine kinase Tyrosine-protein kinase receptor EPH-2 |
| Molecular Weight(MW) | 109885 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2 In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively |
| Tissue Specificity | Preferentially expressed in brain. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite (By similarity) |
| Entrez Gene | 2047 |
|---|
Application
Western blot analysis of EphB1 (arrow) using rabbit polyclonal EphB1 Antibody 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
