Application
Figure 1: Western blot analysis using anti-EhpB1 monoclonal antiobdy against MDA-MB-468(1), MDA-MB-453(2), MCF-7(3), T47D(4) and SKBR-3(5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and colon cancer(right) showing cytoplasmic localization with DAB staining using anti-EphB1 antibody.| Product Name | EhpB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F10A4 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ELK, EPHT2, HEK6, NET |
|---|---|
| Alternative Names | ELK NET Hek6 EPHT2 EPHB1 |
| Molecular Weight(MW) | 109885 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2 In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively |
| Tissue Specificity | Preferentially expressed in brain. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite (By similarity) |
| Entrez Gene | 2047 |
|---|---|
| Nucleotide Accession | NP_004432.1 |
Application
Figure 1: Western blot analysis using anti-EhpB1 monoclonal antiobdy against MDA-MB-468(1), MDA-MB-453(2), MCF-7(3), T47D(4) and SKBR-3(5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and colon cancer(right) showing cytoplasmic localization with DAB staining using anti-EphB1 antibody.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EphB1(aa19-133) expressed in E. Coli. |
