Application
Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 1A10A10 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:2000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAPD |
|---|---|
| Alternative Names | GAPDH GAPD Glyceraldehyde-3-phosphate dehydrogenase Peptidyl-cysteine S-nitrosylase GAPDH |
| Molecular Weight(MW) | 36053 Da |
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation |
| Tissue Specificity | GAPDH recombinant protein is used to produce this monoclonal antibody. |
| Cellular Localization | Cytoplasm, cytosol. Nucleus (By similarity). Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton (By similarity). Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions |
| Entrez Gene | 2597 |
|---|
Application
Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.| Application Notes | WB:1:2000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
