Application
Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020a) in HepG2,A375,A2058,NCI-H460 and CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the Mab ascites (1:25000 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH Monoclonal Antibody (Cat.#AM1020a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GAPDH Antibody (Ascites) (Cat#AM1020a) with hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GAPDH Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 1A10A10 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:5000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GAPD |
|---|---|
| Alternative Names | GAPDH GAPD Glyceraldehyde-3-phosphate dehydrogenase Peptidyl-cysteine S-nitrosylase GAPDH |
| Molecular Weight(MW) | 36053 Da |
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation |
| Tissue Specificity | GAPDH recombinant protein is used to produce this monoclonal antibody. |
| Cellular Localization | Cytoplasm, cytosol. Nucleus (By similarity). Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton (By similarity). Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions |
| Entrez Gene | 2597 |
|---|
Application
Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020a) in HepG2,A375,A2058,NCI-H460 and CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the Mab ascites (1:25000 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH Monoclonal Antibody (Cat.#AM1020a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GAPDH Antibody (Ascites) (Cat#AM1020a) with hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:5000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
