Application
GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).Application
GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GPHN Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPHN; GPH; KIAA1385; Gephyrin; Molybdopterin adenylyltransferase; Domain G; Molybdopterin molybdenumtransferase; Domain E |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPH KIAA1385 |
|---|---|
| Alternative Names | GPHN GPH KIAA1385 Gephyrin Molybdopterin adenylyltransferase Domain G Molybdopterin molybdenumtransferase Domain E |
| Molecular Weight(MW) | 79748 Da |
| Function | Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released |
| Tissue Specificity | This GPHN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444~474 amino acids from the Central region of human GPHN. |
| Cellular Localization | Cell junction, synapse (By similarity). Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Cytoplasmic face of glycinergic postsynaptic membranes (By similarity) |
| Entrez Gene | 10243 |
|---|
Application
GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).
Application
GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
