Application
Figure 1: Western blot analysis using anti-HAND1 monoclonal antibody against truncated Trx-HAND1 recombinant protein(1).| Product Name | HAND1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hxt; eHand; Thing1; bHLHa27 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8E7A11,8C2F4 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHA27 EHAND |
|---|---|
| Alternative Names | Hxt eHand Thing1 bHLHa27 |
| Molecular Weight(MW) | 23627 Da |
| Function | Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box) (By similarity) |
| Tissue Specificity | Heart. |
| Cellular Localization | Nucleus, nucleoplasm (By similarity). Nucleus, nucleolus (By similarity). Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity) |
| Entrez Gene | 9421 |
|---|---|
| Nucleotide Accession | NP_004812.1 |
Application
Figure 1: Western blot analysis using anti-HAND1 monoclonal antibody against truncated Trx-HAND1 recombinant protein(1).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of HAND1(aa90-190) expressed in E. Coli. |
