Application
HSPA1A antibody (Cat. #AM1877b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).Application
Confocal immunofluorescent analysis of HSPA1A Antibody (Cat#AM1877b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | HSPA1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA1A; HSPA1; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 201CT8.2.3 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~2000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSPA1 |
|---|---|
| Alternative Names | HSPA1A HSPA1 Heat shock 70 kDa protein 1A/1B Heat shock 70 kDa protein 1/2 |
| Molecular Weight(MW) | 70052 Da |
| Function | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell |
| Tissue Specificity | HSPA1B is testis-specific. |
| Cellular Localization | Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 3304 |
|---|---|
| Nucleotide Accession | NP_005336.3 |
Application
HSPA1A antibody (Cat. #AM1877b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).
Application
Confocal immunofluorescent analysis of HSPA1A Antibody (Cat#AM1877b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~2000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM235459