Application
HSPA1A (Cat. #AM1877a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).Application
Confocal immunofluorescent analysis of HSPA1A antibody (Ascites)(Cat#AM1877a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | HSPA1A antibody ( Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA1A; HSPA1; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 201CT8.2.3 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500~8000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSPA1 |
|---|---|
| Alternative Names | HSPA1A HSPA1 Heat shock 70 kDa protein 1A/1B Heat shock 70 kDa protein 1/2 |
| Molecular Weight(MW) | 70052 Da |
| Function | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell |
| Tissue Specificity | HSPA1B is testis-specific. |
| Cellular Localization | Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 3304 |
|---|---|
| Nucleotide Accession | NP_005336.3 NP_005337.2 |
Application
HSPA1A (Cat. #AM1877a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).
Application
Confocal immunofluorescent analysis of HSPA1A antibody (Ascites)(Cat#AM1877a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~8000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
Data sheet for OM235461