Application
KPNA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KPNA1 antibody detected the KPNA1 protein (arrow).Application
KPNA1 Antibody (N-term) (AP14416a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KPNA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KPNA1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA1; RCH2; Importin subunit alpha-1; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; RAG cohort protein 2; SRP1-beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RCH2 |
|---|---|
| Alternative Names | KPNA1 RCH2 Importin subunit alpha-1 Karyopherin subunit alpha-1 Nucleoprotein interactor 1 RAG cohort protein 2 SRP1-beta |
| Molecular Weight(MW) | 60222 Da |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS |
| Tissue Specificity | Expressed ubiquitously. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 3836 |
|---|---|
| Nucleotide Accession | NP_002255.3 |
Application
KPNA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KPNA1 antibody detected the KPNA1 protein (arrow).
Application
KPNA1 Antibody (N-term) (AP14416a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KPNA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
