Application
14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma antibody at 1:100 dilution.Application
14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. 14-3-3 sigma antibody dilution: 1:500.Application
14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody 5 % SDS-PAGE The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | 14-3-3 sigma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 sigma (stratifin)Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Chicken, Sheep, Rhesus Monkey, BovinePig (96%), Chicken (80%), Sheep (97%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | YWHAS antibody SFN antibody epithelial cell marker protein 1 antibody 14-3-3 protein sigma antibody 14-3-3 sigma antibody stratifin antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Secreted |
Application
14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
14-3-3 sigma antibody detects SFN protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE 14-3-3 sigma antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma antibody at 1:100 dilution.
Application
14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. 14-3-3 sigma antibody dilution: 1:500.
Application
14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of SFN protein by 4 μg 14-3-3 sigma antibody 5 % SDS-PAGE The immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | A549 , 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
