Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using 14-3-3 sigma antibody at 1:500 dilution.Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody [N1C3-2] diluted at 1:1000.| Product Name | 14-3-3 sigma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 14-3-3 sigma (stratifin)Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 sigma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Pig, Chicken, Sheep, Rhesus Monkey, BovineMouse (98%), Pig (97%), Chicken (81%), Sheep (97%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | YWHAS antibody SFN antibody epithelial cell marker protein 1 antibody 14-3-3 protein sigma antibody 14-3-3 sigma antibody stratifin antibody |
|---|---|
| Target | 14-3-3 sigma |
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Secreted |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using 14-3-3 sigma antibody at 1:500 dilution.
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody [N1C3-2] diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
