Application
Aconitase 1 antibody detects Aconitase 1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Aconitase 1 antibody diluted at 1:500.Application
Aconitase 1 antibody detects Aconitase 1 protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000| Product Name | Aconitase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aconitase 1 (aconitase 1, soluble)Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Aconitase 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, Rhesus Monkey, BovineMouse (87%), Rat (86%), Rabbit (90%), Rhesus Monkey (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | aconitase 1 soluble ACONS IRP1 ACO1 IREBP1 IREBP IREB1 |
|---|---|
| Target | Aconitase 1 |
| Cellular Localization | Cytoplasm |
Application
Aconitase 1 antibody detects Aconitase 1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Aconitase 1 antibody diluted at 1:500.
Application
Aconitase 1 antibody detects Aconitase 1 protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000| Positive Control | A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
