Application
Flow cytometric analysis of Jurkat cells, using anti-AKT2 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.Application
Flow cytometric analysis of Hela cells, using anti-AKT2 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-AKT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-AKT2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody .Application
Anti-AKT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AKT2| Product Name | AKT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4H7] to Akt2General protein kinase capable of phosphorylating several known proteins. |
| Immunogen | Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4H7 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AKT2 RAC-BETA PKBBETA PRKBB RACBETA RAC BETA |
|---|---|
| Target | Akt2 |
| Tissue Specificity | is specific for human AKT2 (NP_001617). |
Application
Flow cytometric analysis of Jurkat cells, using anti-AKT2 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
Application
Flow cytometric analysis of Hela cells, using anti-AKT2 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-AKT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-AKT2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody .
Application
Anti-AKT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AKT2| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
