Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (Phospho-Ser474).Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using Akt2 (Phospho-Ser474).| Product Name | Akt2 (phospho Ser474) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKBB, PRKBB, HIHGHH, PKBBETA, RAC-BETA, IKKA, TCF16, PKB beta, Protein kinase B, RAC-PK-beta |
| Product description | General protein kinase capable of phosphorylating several known proteins.1) Sun M, et al. (2001) Cancer Res; 61 (16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19 (19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272 (48): 30491-7. |
| Immunogen | Akt2 (Phospho-Ser474) antibody was raised against a peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2. |
| Modification | p-Ser474 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT2 |
|---|---|
| Alternative Names | PKBB PRKBB HIHGHH PKBBETA RAC-BETA IKKA TCF16 PKB beta Protein kinase B RAC-PK-beta |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of Akt2 only when phosphorylated at serine 474. |
| Entrez Gene | 208 |
|---|---|
| Protein Accession | NP_001617.1 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (Phospho-Ser474).
Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using Akt2 (Phospho-Ser474).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
