Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of AKT2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | AKT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2 |
| Product description | AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.1) George, S., et al., Science 304(5675):1325-1328 (2004). |
| Immunogen | This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human AKT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AKT2 |
|---|---|
| Alternative Names | RAC-beta serine/threonine-protein kinase Protein kinase Akt-2 Protein kinase B beta PKB beta RAC-PK-beta AKT2 |
| Molecular Weight(MW) | 56 kDa |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of AKT2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
