Application
Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane)Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | AKT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2 |
| Product description | AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.1) Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003). Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003). Jones, D.T., et al., Blood 101(8):3174-3180 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003). |
| Immunogen | This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT2 |
|---|---|
| Alternative Names | RAC-beta serine/threonine-protein kinase Protein kinase Akt-2 Protein kinase B beta PKB beta RAC-PK-beta AKT2 |
| Molecular Weight(MW) | 56 kDa |
Application
Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane)
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
