Application
Immunofluorescent analysis of alpha Adaptin in HeLa cells. alpha Adaptin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with alpha Adaptin antibody [AP6] at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.| Product Name | alpha Adaptin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AP6] to alpha AdaptinThis gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified adaptors. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AP6 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFACSICC/IFIHCIPWB |
| ICC/IF: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouseDrosophilaRatHamsterPrimateBovineInvertebrates |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | adaptor-related protein complex 2 alpha 1 subunit CLAPA1 AP2A1 AP2-ALPHA ADTAA |
|---|---|
| Target | alpha Adaptin |
| Tissue Specificity | This antibody detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha adaptin found in neurons. |
Application
Immunofluorescent analysis of alpha Adaptin in HeLa cells. alpha Adaptin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with alpha Adaptin antibody [AP6] at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.| Application Notes | ICC/IF: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide |
