Application
Western blot analysis using CRYAB mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).Application
Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D), showing cytoplasmic localization with DAB staining using anti-CRYAB mAb.Application
Western blot analysis using anti-CRYAB mAb against full-length CRYAB recombinant protein(1).Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using anti-CRYAB mAb.| Product Name | alpha B Crystallin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D11C6E6] to CRYABCrystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular c |
| Immunogen | The immunogen corresponding to a region within amino acids 1 and 175 of CRYAB expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1D11C6E6 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CTPP2 HSPB5 CRYA2 CMD1II |
|---|---|
| Target | CRYAB |
| Cellular Localization | Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
Application
Western blot analysis using CRYAB mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).
Application
Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D), showing cytoplasmic localization with DAB staining using anti-CRYAB mAb.
Application
Western blot analysis using anti-CRYAB mAb against full-length CRYAB recombinant protein(1).
Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using anti-CRYAB mAb.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
