Application
Immunofluorescence staining of methanol-fixed Hela cells using alpha -Synuclein (Phospho-Tyr133) Antibody .Application
Western blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using a-Synuclein (Phospho-Tyr133) Antibody| Product Name | alpha Synuclein (phospho Tyr133) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Synuclein (Phospho Tyr133)SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 133 (E-G-Y(p)-Q-D) derived from Human alpha Synuclein. |
| Modification | p-Tyr133 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC110988 NACP PARK1 PARK4 PD1 SNCA non A-beta component of AD amyloid alpha-synuclein alpha-synuclein isoform NACP140 synuclein alpha-140 synuclein alpha (non A4 component of amyloid precursor) |
|---|---|
| Target | alpha Synuclein (Phospho Tyr133) |
| Molecular Weight(MW) | 18 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using alpha -Synuclein (Phospho-Tyr133) Antibody .
Application
Western blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using a-Synuclein (Phospho-Tyr133) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
