Application
Immunofluorescence staining of methanol-fixed Hela cells using alpha -Synuclein (Phospho-Tyr136) Antibody .Application
Western blot analysis of extracts from mouse brain tissue using a-Synuclein(Phospho-Tyr136) Antibody and the same antibody preincubated with blocking peptide.| Product Name | alpha Synuclein (phospho Tyr136) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Synuclein (Phospho Tyr136)May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 136 (Q-D-Y(p)-E-P) derived from Human alpha Synuclein. |
| Modification | p-Tyr136 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC110988 NACP PARK1 PARK4 PD1 SNCA non A-beta component of AD amyloid alpha-synuclein alpha-synuclein isoform NACP140 synuclein alpha-140 synuclein alpha (non A4 component of amyloid precursor) |
|---|---|
| Target | alpha Synuclein (Phospho Tyr136) |
| Molecular Weight(MW) | 18 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using alpha -Synuclein (Phospho-Tyr136) Antibody .
Application
Western blot analysis of extracts from mouse brain tissue using a-Synuclein(Phospho-Tyr136) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
