Application
Human Brain stained with anti-Synuclein-alpha antibody| Product Name | alpha Synuclein antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to alpha Synuclein predilutedThe implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimer's disease (AD) brains. There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinson's disease, and Alzheimer's disease. Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinson's disease (PD). Alpha Synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD. Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes. The synuclein phosphoproteins (15-20 kD) are small highly conserved proteins in vertebrates. The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin. The expression is abundant in neurons and typically is localized at presynaptic terminals. Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SNCA PD1 NACP PARK4 6622 MGC110988 P37840 127750 PARK1 alpha Synuclein |
|---|---|
| Target | alpha Synuclein |
| Tissue Specificity | Specific for alpha synuclein. Does not cross react with beta synuclein. |
| Cellular Localization | Cytoplasmic and Nuclear. Membrane-bound in dopaminergic neurons. |
Application
Human Brain stained with anti-Synuclein-alpha antibody| Positive Control | Posterior cingulated gyrus, stains neuronal cytosol and presynaptic terminals |
|---|---|
| Application Notes | IHC-P: Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
