Application
Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin ( green) and anti-Vimentin ( red). Nucleus is stained with DAPI (blue)Application
ICC/IF analysis of 3T3 cells using alpha Tubulin antibody [TU-01] (green) and DAPI (blue).Application
IP of alpha Tubulin from HeLa and Raji lysates by alpha Tubulin antibody [TU-16] was performed. The precipitated protein was detected by alpha Tubulin antibody [TU-01].Application
Use of anti-alpha-tubulin antibody a loading control (A) in an Western blot experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B)Application
Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin ( red). Nucleus is stained with DAPI (blue).| Product Name | alpha Tubulin antibody [TU-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [TU - 01] to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin. |
| Immunogen | Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TU-01 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| ELISA: Use at an assay dependant dilution. ICC: Use at a dilution of 1/100. For ICC, we recommend not only fixing but also permeabilizing the cells. IF: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. GTX27749 (TU-16)is better for IP application. WB: Use at an assay dependant dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Mammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBA1 Tubulin alpha 1 chain Tubulin alpha |
|---|---|
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
Application
Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin ( green) and anti-Vimentin ( red). Nucleus is stained with DAPI (blue)
Application
ICC/IF analysis of 3T3 cells using alpha Tubulin antibody [TU-01] (green) and DAPI (blue).
Application
IP of alpha Tubulin from HeLa and Raji lysates by alpha Tubulin antibody [TU-16] was performed. The precipitated protein was detected by alpha Tubulin antibody [TU-01].
Application
Use of anti-alpha-tubulin antibody a loading control (A) in an Western blot experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B)
Application
Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin ( red). Nucleus is stained with DAPI (blue).| Application Notes | ELISA: Use at an assay dependant dilution. ICC: Use at a dilution of 1/100. For ICC, we recommend not only fixing but also permeabilizing the cells. IF: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. GTX27749 (TU-16)is better for IP application. WB: Use at an assay dependant dilution. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
